Part:BBa_K228866:Design

AND Gate pBad-SupD pSal-PhiR73 delta(amber mutation)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1274
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2007
  Illegal BamHI site found at 1214
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1839
  Illegal AgeI site found at 1320
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1323
  

Design Notes

when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.

Source

part registery 2009 distribution & PKU 2009 igem team